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Research Papers

Development of a Multifunction Isolator System for the Care of Medium-Sized Laboratory Animals Harboring Infectious Diseases

[+] Author and Article Information
Xin Pan1

Animal Biosafety Level 3 Laboratory, Second Military Medical University, 8 Pan Shan Road, Shanghai 200433, Chinaxinpanpx@163.com

Ming Long

Animal Biosafety Level 3 Laboratory, Second Military Medical University, 8 Pan Shan Road, Shanghai 200433, Chinapaullong@topleader.com.cn

Hao Liang

Animal Biosafety Level 3 Laboratory, Second Military Medical University, 8 Pan Shan Road, Shanghai 200433, Chinahaozihaozi_2004@163.com

Xiao Chen

Animal Biosafety Level 3 Laboratory, Second Military Medical University, 8 Pan Shan Road, Shanghai 200433, Chinaciwawayang@163.com

Han Li

Animal Biosafety Level 3 Laboratory, Second Military Medical University, 8 Pan Shan Road, Shanghai 200433, Chinalihan19840510@126.com

Guang-bo Li

Animal Biosafety Level 3 Laboratory, Second Military Medical University, 8 Pan Shan Road, Shanghai 200433, Chinaliguangsd@126.com

Zi-ye Zhao

Animal Biosafety Level 3 Laboratory, Second Military Medical University, 8 Pan Shan Road, Shanghai 200433, Chinayemenzhao@sina.com

1

Corresponding author.

J. Med. Devices 4(4), 041004 (Nov 08, 2010) (5 pages) doi:10.1115/1.4002623 History: Received June 22, 2010; Revised July 01, 2010; Published November 08, 2010; Online November 08, 2010

The close phylogenetic relationship between humans and nonhuman primates makes nonhuman primates an irreplaceable model for the study of human infectious diseases. In this study, we describe the development of an automatic multifunctional isolation chamber for use with medium-sized laboratory animals carrying an infectious disease. The isolator system, including the transfer chain, disinfection chain, negative air pressure isolation system, animal welfare system, and the automated system, was designed to meet all biological safety standards. To create an internal chamber environment that was completely isolated from the exterior, variable frequency drive blowers were used in the air-intake and air-exhaust system, precisely controlling the filtered airflow and providing an air-barrier protection. To transfer the material between the interior of the isolation chamber and the outside, a double door transfer port was used. A peracetic acid sterilizer and its affiliated pipeline allowed for a complete disinfection of the isolation chamber. All of the isolator system parameters can be automatically controlled by a programmable computerized menu allowing for work with different animals in different sized cages depending on the research project. The multifunctional isolator system should be a useful safety device for working with medium-sized laboratory infectious animals in high-level biosafety laboratories.

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Copyright © 2010 by American Society of Mechanical Engineers
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Figure 1

Negative-pressure isolation system structural diagram. (1) Top ventilation unit, (2) isolation chamber working zone, (3) lower part of the control system, (4) isolation chamber support stand, (5) control cabinet, (6) damping-brace for the front door, (7) front door, (8) sterilization bypass pipe, (9) exhaust ventilation pipe, (10) exhaust electronic control ball valve, (11) exhaust two-in-series HEPA-filters, (12) sterilization bypass electric control ball valve, (13) exhaust export, (14) anemometer, (15) extraction blower, (16) inlet air blower, (17) inlet ventilation pipe, (18) inlet HEPA filter, (19) inlet air electronic control ball valve, (20) coupling clamp for inlet ventilation pipe, (21) stainless-steel box, (22) top installation slideway, (23) temperature humidity sensor, (24) microdifferential pressure sensor, (25) sterilization reagent import, (26) alarm indicator light tower, (27) control touch panel, (28) camera installed base, (29) rotatable camera, (30) DPTE 350 α door, (31) manual water inlet valve, (32) DPTE 270 transfer bin container, (33) transfer bin container HEPA filter, (34) cage slideway and pulley, (35) drain valve, (36) disinfection pool, (37) animal cage, (38) glove and sleeve system, (39) adjustable illumination lamp, (40) television-installed box, and (41) flat television.

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