Intracytoplasmic sperm injection has attracted research interest from both biological and engineering groups. The technology is constantly evolving to perform this procedure with precision and speed. One such development is the contribution of this paper. We focus on a relatively recent procedure called Ros-Drill© (rotationally oscillating drill), of which the early versions have already been effectively utilized for the mice. Here, we present a procedure to automate a critical part of the operation: initiation of the rotational oscillation. Visual feedback is used to track the pipette tip. Predetermined species-specific penetration depth is successfully utilized to initiate the rotational oscillation command. Penetration-depth-based decisions concur with our earlier curvature-based approach.