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Technical Briefs

Development of a Portable Tissue Micro Array Instrument

[+] Author and Article Information
K. K. Tan

Department of Electrical and Computer Engineering,  National University of Singapore, Singapore, 117576kktan@nus.edu.sg

A. S. Putra

Department of Electrical and Computer Engineering,  National University of Singapore, Singapore, 117576elepas@nus.edu.sg

L. P. Pham

Department of Electrical and Computer Engineering,  National University of Singapore, Singapore, 117576le4phuong@yahoo.com

T. H. Lee

Department of Electrical and Computer Engineering,  National University of Singapore, Singapore, 117576eleleeth@nus.edu.sg

M. Salto-Tellez

 Queen’s University of Belfast, 97 Lisburn Road, Belfast, United Kingdom, BT97BLsaltotellez@hotmail.com

L. G. Kim

Department of Pathology,  National University of Singapore, Singapore, 119047csiklg@nus.edu.sg

J. Med. Devices 5(4), 044503 (Nov 14, 2011) (7 pages) doi:10.1115/1.4004922 History: Received May 27, 2010; Revised August 10, 2011; Published November 14, 2011

Tissue micro array (TMA) is based on the idea of applying miniaturization and a high throughput approach to hybridization-based analyses of tissues. It facilitates biomedical research on a large scale in a single experiment; thus representing one of the most commonly used technologies in translational research. A critical analysis of the existing TMA instruments indicates that there are potential constraints in terms of portability, apart from costs and complexity. This paper will present the development of an affordable, configurable, and portable TMA instrument to allow an efficient collection of tissues, especially in instrument-to-tissue scenarios. The purely mechanical instrument requires no energy sources other than the user, is light weight, portable, and simple to use.

Copyright © 2011 by American Society of Mechanical Engineers
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References

Figures

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Figure 19

Sample array of tissues obtained with instrument

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Figure 20

Paraffin pieces cut from the donor (5 left bars) and recipient (5 right bars) blocks

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Figure 21

Images of sample pieces cut from the blocks by the prototype (the left set) and the commercial device (the right set)

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Figure 1

Front and left view of the instrument

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Figure 3

Telescopic design for tissue and paraffin purging

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Figure 4

Front and back part of a punch sleeve

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Figure 5

A punch sleeve, two different size needles and end interface

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Figure 6

Precision depth control mechanism

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Figure 7

Support and stabilizing structure

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Figure 8

Portable platform to maintain orthogonality

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Figure 9

Stress (on the left side) and displacement distribution (axial strain) in P1

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Figure 10

Stress (on the left side) and displacement distribution (buckling strain) in P2

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Figure 11

TMA process using the instrument

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Figure 12

Front view of the prototype

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Figure 13

Side view of the prototype

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Figure 14

Snug fit of the instrument in the hand

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Figure 15

Donor/recipient members

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Figure 16

Support and stabilizing structure

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Figure 17

(left) Inserting the donor member into the donor block and removing tissue. (right) Inserting the recipient member into the recipient block and creating a void.

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Figure 18

Releasing the tissue sample to the void in the recipient block

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