Technical Briefs

Characterization of Puncture Forces for Retinal Vein Cannulation

[+] Author and Article Information
Olgaç Ergeneman

 Institute of Robotics and Intelligent Systems, ETH Zurich, 8092 Zurich, Switzerlandoergeneman@ethz.ch

Juho Pokki, Vanda Počepcová, Bradley J. Nelson

 Institute of Robotics and Intelligent Systems, ETH Zurich, 8092 Zurich, Switzerland

Heike Hall

 Cells and BioMaterials, Department of Materials, ETH Zurich, 8093 Zurich, Switzerland

Jake J. Abbott

 Department of Mechanical Engineering, University of Utah, Salt Lake City, Utah, 84112

J. Med. Devices 5(4), 044504 (Nov 15, 2011) (6 pages) doi:10.1115/1.4005318 History: Received March 29, 2011; Revised September 02, 2011; Published November 15, 2011; Online November 15, 2011

For this study, we have collected puncture force data from the vasculature of the chorioallantoic membranes (CAM) of developing chicken embryos to examine forces required for retinal vein cannulation. The CAM vessels of a developing chicken embryo have been shown to be an appropriate model for human retinal veins. The effect of microneedle geometry and vessel size on puncture forces was investigated. The results of this work are important for researchers working on robotic vitreoretinal surgical systems.

Copyright © 2011 by American Society of Mechanical Engineers
Your Session has timed out. Please sign back in to continue.



Grahic Jump Location
Figure 1

Two types of microneedles were prepared: blunt and beveled. The outer diameter (OD) and bevel angle is shown in the image.

Grahic Jump Location
Figure 2

Setup for puncture-force experiments. A microneedle is attached on a force sensor, which is mounted on a micromanipulator. The puncture events were observed using a microscope and images were captured by a camera. On the top left, a microneedle advancing toward a blood vessel is shown. The microneedle was mounted at 45° from the normal of the plane of the petri dish and was advanced in its axial direction. The petri dish was oriented to have the vessel axis perpendicular to the microneedle axis.

Grahic Jump Location
Figure 3

Puncture force (F1) and the phases of the puncture-force experiment are shown. The compressive force on the sensor is shown as negative force. The steps in plot (a) are due to the movement of the stepper motor, and they are not seen in (b) because the magnitude of forces is higher.

Grahic Jump Location
Figure 4

Histogram of magnitude of forces as percentages of all measurements. Vessels in 80–400 μm OD range were considered.

Grahic Jump Location
Figure 5

Average puncture forces for 1-2 μm, 9–15 μm, 29–34 μm, 46–51 μm, and 70–73 μm tip OD for the blunt microneedles (306 individual punctures)

Grahic Jump Location
Figure 6

Average puncture forces for 13–15 μm, 25 μm, 46–52 μm, and 68–69 μm tip OD for the beveled microneedles (153 individual punctures)

Grahic Jump Location
Figure 7

Residual analysis for the regression model using the force data with blunt needles

Grahic Jump Location
Figure 8

Residual analysis for the regression model using the force data with beveled needles



Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related Journal Articles
Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In