Research Paper

Computer-Aided 4D Modeling of Hydrolytic Degradation in Micropatterned Bioresorbable Membranes

[+] Author and Article Information
Ibrahim T. Ozbolat

Department of Mechanical and Industrial Engineering,
Biomanufacturing Laboratory,
Center for Computer-Aided Design,
The University of Iowa,
Iowa, IA 52242
e-mail: ibrahim-ozbolat@uiowa.edu

Joseph A. Gardella, Jr.

Department of Chemistry,
University at Buffalo,
359 Natural Sciences Complex,
Buffalo, NY 14260

Bahattin Koc

Faculty of Engineering and Natural Sciences,
Sabanci University,
FENS G013 Tuzla,
Istanbul 34956, Turkey

1Corresponding author.

Manuscript received June 3, 2012; final manuscript received February 27, 2013; published online June 24, 2013. Assoc. Editor: Erol Sancaktar.

J. Med. Devices 7(2), 021004 (Jun 24, 2013) (9 pages) Paper No: MED-12-1077; doi: 10.1115/1.4024158 History: Received June 03, 2012; Revised February 27, 2013

Real-time degradation studies of bioresorbable polymers can take weeks, months, and even years to conduct. For this reason, developing and validating mathematical models that describe and predict degradation can provide a means to accelerate the development of materials and devices for controlled drug release. This study aims to develop and experimentally validate a computer-aided model that simulates the hydrolytic degradation kinetics of bioresorbable polymeric micropatterned membranes for tissue engineering applications. Specifically, the model applies to circumstances that are conducive for the polymer to undergo surface erosion. The developed model provides a simulation tool enabling the prediction and visualization of the dynamic geometry of the degrading membrane. In order to validate the model, micropatterned polymeric membranes were hydrolytically degraded in vitro and the morphological changes were analyzed using optical microscopy. The model is then extended to predict spatiotemporal degradation kinetics of variational micropatterned architectures.

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Fig. 1

(a) Photograph of a manufactured silicon wafer using photolithography with a block size of 200 × 200 × 5 μm, and (b) the DIC image of a fabricated PLA micropatterned membrane

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Fig. 2

A micropatterned membrane with diffusion directions and (m,n,s) number of unit cells on a block

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Fig. 7

(a) SEM image of a fabricated PLLA membrane with uniform 200 × 200 × 5 μm blocks, and (b) degraded micropatterned structure in pH 7 potassium phosphate buffer solution after 3 days (scale bar shows 200 μm)

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Fig. 6

Daily DIC images of the fabricated micropatterned structure with uniform 200 × 200 × 5 μm blocks: (a) a block at hour 0 with an originally square shape, (b) the etching effect observed at the corners after 24 h of degradation, (c) and (d) followed by further etching at the end of the second day, and the (e) and (f) images show the degradation results at the end of the third day (scale bar shows 100 μm)

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Fig. 5

Degradation profile for nine volumetrically equal patterns presented in Table 1

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Fig. 4

Degradation profiles of three different blocks with height values of h, 2h, and 4h with a fixed width w for a time increment t

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Fig. 8

A micropatterned membrane example with (a) 2 PFs: starting PF (2 edges) and ending PF (1 point), (b) spatial variation of total volume of material released, and (c) corresponding time-based degradation kinetics

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Fig. 3

(a) Isometric view of the degrading block, and (b) top and side views of the degradation process based on 24 h time increments for a single PLA block with the dimensions of 200 × 200 × 5 μm



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