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Research Papers

Blood Cell Adhesion on a Polymeric Heart Valve Leaflet Processed Using Magnetic Abrasive Finishing

[+] Author and Article Information
Hitomi Yamaguchi

e-mail: hitomiy@ufl.edu

Department of Mechanical and
Aerospace Engineering,
University of Florida,
Gainesville, FL 32611

Faris Al-Mousily

Department of Pediatrics,
University of Florida,
Gainesville, FL 32611

Curt DeGroff

Congenital Heart Center,
University of Florida,
Gainesville, FL 32611

Manuscript received February 25, 2013; final manuscript received October 6, 2013; published online December 6, 2013. Assoc. Editor: Keefe B. Manning.

J. Med. Devices 8(1), 011005 (Dec 06, 2013) (8 pages) Paper No: MED-13-1021; doi: 10.1115/1.4025853 History: Received February 25, 2013; Revised October 06, 2013

Polymeric heart valves have the potential to improve hemodynamic function without the complications associated with bioprosthetic and mechanical heart valves, but they have exhibited issues that need to be addressed including calcification, hydrolysis, low durability, and the adhesion of blood cells on the valves. These issues are attributed to the valves' material properties and surface conditions in addition to the hemodynamics. To overcome these issues, a new stentless, single-component trileaflet polymeric heart valve with engineered leaflet surface texture was designed, and prototypes were fabricated from a simple polymeric tube. The single-component structure features a trileaflet polymeric valve and conduit that are made of a single tube component to eliminate complications possibly caused by the interaction of multiple materials and components. This paper focuses on the leaflet surface modification and the effects of leaflet surface texture on blood cell adhesion to the leaflet surface. Silicone rubber was chosen as the working material. A magnetic abrasive finishing (MAF) process was used to alter the inner surface of the tubular mold in contact with the silicone leaflets during the curing process. It was hypothesized that the maximum profile height Rz of the mold surface should be smaller than the minimum platelet size of 1 μm to prevent platelets (1–3 μm in diameter) from becoming lodged between the peaks. Cell adhesion studies using human whole blood flushed at low shear stresses over leaflet surfaces with six different textures showed that adhesion of the platelets and red blood cells is greatly influenced by both surface roughness and lay. Leaflets replicated from MAF-produced mold surfaces consisting of short asperities smaller than 1 μm reduced blood cell adhesion and aggregation. Cell adhesion studies also found that either mold or leaflet surface roughness can be used as a measure of cell adhesion.

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Figures

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Fig. 1

Photographs of porcine pulmonary valve and polymeric valve

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Fig. 8

Blood cell adhesion test schematic and photograph of experimental setup

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Fig. 7

Three-dimensional surface shapes of leaflet 6 cured in air (0.42 μm Sz, 0.07 μm Sa)

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Fig. 6

Three-dimensional surface shapes of mold 4a and leaflet 4a measured by optical profiler

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Fig. 5

Leaflet roughness Sa with mold roughness Ra

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Fig. 4

Mold surface roughness profiles

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Fig. 3

Processing principle and finishing machine

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Fig. 2

Average thickness of silicone leaflets with mold revolution

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Fig. 9

Microscopic images (170 μm × 220 μm) of blood cell adhesion on leaflet 2 (with grid)

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Fig. 10

Average number of platelets adhered to silicone leaflets

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Fig. 11

Relationship between average numbers of platelets and red blood cells adhered to leaflet and leaflet roughness

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Fig. 12

Relationship between average numbers of platelets and red blood cells adhered to leaflet and mold roughness

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