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Research Papers

Blood Cell Adhesion on a Polymeric Heart Valve Leaflet Processed Using Magnetic Abrasive Finishing

[+] Author and Article Information
Hitomi Yamaguchi

e-mail: hitomiy@ufl.edu

Department of Mechanical and
Aerospace Engineering,
University of Florida,
Gainesville, FL 32611

Faris Al-Mousily

Department of Pediatrics,
University of Florida,
Gainesville, FL 32611

Curt DeGroff

Congenital Heart Center,
University of Florida,
Gainesville, FL 32611

Manuscript received February 25, 2013; final manuscript received October 6, 2013; published online December 6, 2013. Assoc. Editor: Keefe B. Manning.

J. Med. Devices 8(1), 011005 (Dec 06, 2013) (8 pages) Paper No: MED-13-1021; doi: 10.1115/1.4025853 History: Received February 25, 2013; Revised October 06, 2013

Polymeric heart valves have the potential to improve hemodynamic function without the complications associated with bioprosthetic and mechanical heart valves, but they have exhibited issues that need to be addressed including calcification, hydrolysis, low durability, and the adhesion of blood cells on the valves. These issues are attributed to the valves' material properties and surface conditions in addition to the hemodynamics. To overcome these issues, a new stentless, single-component trileaflet polymeric heart valve with engineered leaflet surface texture was designed, and prototypes were fabricated from a simple polymeric tube. The single-component structure features a trileaflet polymeric valve and conduit that are made of a single tube component to eliminate complications possibly caused by the interaction of multiple materials and components. This paper focuses on the leaflet surface modification and the effects of leaflet surface texture on blood cell adhesion to the leaflet surface. Silicone rubber was chosen as the working material. A magnetic abrasive finishing (MAF) process was used to alter the inner surface of the tubular mold in contact with the silicone leaflets during the curing process. It was hypothesized that the maximum profile height Rz of the mold surface should be smaller than the minimum platelet size of 1 μm to prevent platelets (1–3 μm in diameter) from becoming lodged between the peaks. Cell adhesion studies using human whole blood flushed at low shear stresses over leaflet surfaces with six different textures showed that adhesion of the platelets and red blood cells is greatly influenced by both surface roughness and lay. Leaflets replicated from MAF-produced mold surfaces consisting of short asperities smaller than 1 μm reduced blood cell adhesion and aggregation. Cell adhesion studies also found that either mold or leaflet surface roughness can be used as a measure of cell adhesion.

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References

Yoganathan, A., He, Z., and Jones, S., 2004, “Fluid Mechanics of Heart Valves,” Annu. Rev. Biomed. Eng., 6, pp. 331–362. [CrossRef] [PubMed]
Dasi, L. P., Simon, H. A., Sucosky, P., and Yoganathan, A. P., 2009, “Fluid Mechanics of Artificial Heart Valves,” Clin. Exp. Pharmacol. Physiol., 36, pp. 225–237. [CrossRef] [PubMed]
Edmunds, L. H., Mckinlay, S., Anderson, J. M., Callahan, T. H., Chesebro, J. H., Geiser, E. A., Makanani, D. M., McIntire, L. V., Meeker, W. Q., Naughton, G. K., Panza, J. A., Schoen, F. J., and Didisheim, P., 1997, “Directions for Improvement of Substitute Heart Valves: National Heart, Lung, and Blood Institute's Working Group Report on Heart Valves,” J. Biomed. Mater. Res., 38(3), pp. 263–266. [CrossRef] [PubMed]
Zilla, P., Brink, J., Human, P., and Bezuidenhout, D., 2008, “Prosthetic Heart Valves: Catering for the Few,” Biomaterials, 29(4), pp. 385–406. [CrossRef] [PubMed]
Sun, J. C., Davidson, M. J., Lamy, A., and Eikelboom, J. W., 2009, “Antithrombotic Management of Patients With Prosthetic Heart Valves: Current Evidence and Future Trends,” Lancet, 374(9689), pp. 565–576. [CrossRef] [PubMed]
Zadeh, P. B., 2009, “Calcification of Polyurethane Heart Valve Prosthesis,” M.S. thesis, Northeastern University, Boston, MA.
Colas, A., and Curtis, J., 2004, “Silicone Biomaterials: History and Chemistry,” Biomaterials Science: An Introduction to Materials in Medicine, 2nd ed., D. R.Ratner, A. S.Hoffman, F. J.Schoen, and J. E.Lemons, eds., Elsevier, New York, pp. 80–85.
Ghanbari, H., Viatge, H., Kidane, A. G., Burriesci, G., Tavakoli, M., and Seifalian, A. M., 2009, “Polymeric Heart Valves: New Materials, Emerging Hopes,” Trends Biotechnol., 27(6), pp. 359–367. [CrossRef] [PubMed]
Zdrahala, R. J., and Zdrahala, I. J., 1999, “Biomedical Applications of Polyurethanes: A Review of Past Promises, Present Realities, and a Vibrant Future,” J. Biomater. Appl., 14(1), pp. 67–90. [CrossRef] [PubMed]
Sachweh, J. S., and Daebritz, S. H., 2006, “Novel ‘Biomechanical’ Polymeric Valve Prostheses With Special Design for Aortic and Mitral Position: A Future Option for Pediatric Patients?,” ASAIO J., 52(5), pp. 575–580. [CrossRef] [PubMed]
Daebritz, S. H., Sachweh, J. S., Hermanns, B., Fausten, B., Franke, A., Groetzner, J., Klosterhalfen, B., and Messmer, B. J., 2003, “Introduction of a Flexible Polymeric Heart Valve Prosthesis With Special Design for Mitral Position,” Circulation, 108, pp. II-134–II-139. [CrossRef]
Kidane, A. G., Burriesci, G., Edirisinghe, M., Ghanbari, H., Bonhoeffer, P., and Seifalian, A. M., 2009, “A Novel Nanocomposite Polymer for Development of Synthetic Heart Valve Leaflets,” Acta Biomater., 5(7), pp. 2409–2417. [CrossRef] [PubMed]
Mohammadi, H., and Mequanint, K., 2011, “Prosthetic Aortic Heart Valves: Modeling and Design,” Med. Eng. Phys., 33(2), pp. 131–147. [CrossRef] [PubMed]
Korossis, S. A., Fisher, J., and Ingham, E., 2000, “Cardiac Valve Replacement: A Bioengineering Approach,” Biomed. Mater. Eng., 10, pp. 83–124. [PubMed]
Bach, D. S., David, T., Yacoub, M., Pepper, J., Goldman, B., Wood, J., Verrier, E., Petracek, M., Aldrete, V., Rosenbloom, M., Azar, H., and Rakowski, H., 1998, “Hemodynamics and Left Ventricular Mass Regression Following Implantation of the Toronto SPV Stentless Porcine Valve,” Am. J. Cardiol., 82(10), pp. 1214–1219. [CrossRef] [PubMed]
Mohandas, N., Hochmuth, R. M., and Spaeth, E. E., 1974, “Adhesion of Red Cells to Foreign Surfaces in the Presence of Flow,” J. Biomed. Mater. Res., 8(2), pp. 119–136. [CrossRef] [PubMed]
Milner, K. R., Siedlecki, C. A., and Snyder, A. J., 2005, “Development of Novel Submicron Textured Polyether (Urethane Urea) for Decreasing Platelet Adhesion,” ASAIO J., 51(5), pp. 578–584. [CrossRef] [PubMed]
Martines, E., McGhee, K., Wilkinson, C., and Curtis, A., 2004, “A Parallel-Plate Flow Chamber to Study Initial Cell Adhesion on a Nanofeatured Surface,” IEEE Trans. Nanobiosci., 3(2), pp. 90–95. [CrossRef]
Hallab, N. J., Bundy, K. J., O'Connor, K., Moses, R. L., and Jacobs, J. J., 2001, “Evaluation of Metallic and Polymeric Biomaterial Surface Energy and Surface Roughness Characteristics for Directed Cell Adhesion,” Tissue Eng., 7(1), pp. 55–71. [CrossRef] [PubMed]
Thapa, A., Webster, T., and Haberstroh, K., 2003, “Polymers With Nano-Dimensional Surface Features Enhance Bladder Smooth Muscle Cell Adhesion,” J. Biomed. Mater. Res., 67A(4), pp. 1374–1383. [CrossRef]
Stavridi, M., Katsikogianni, M., and Missirlis, Y. F., 2003, “The Influence of Surface Patterning and/or Sterilization on the Haemocompatibility of Polycaprolactones,” Mater. Sci. Eng., C, 23(3), pp. 359–365. [CrossRef]
Kuwahara, M., Sugimoto, M., Tsuji, S., Matsui, H., Mizuno, T., Miyata, S., and Yoshioka, A., 2002, “Platelet Shape Changes and Adhesion Under High Shear Flow,” Arterioscler., Thromb., Vasc. Biol., 22(2), pp. 329–334. [CrossRef]
Park, J. Y., Gemmell, C. H., and Davies, J. E., 2001, “Platelet Interactions With Titanium: Modulation of Platelet Activity by Surface Topography,” Biomaterials, 22(19), pp. 2671–2682. [CrossRef] [PubMed]
Curtis, A., and Wilkinson, C., 1997, “Topographical Control of Cells,” Biomaterials, 18(24), pp. 1573–1583. [CrossRef] [PubMed]
Milner, K., and Siedlecki, C., 2007, “Fibroblast Response is Enhanced by Poly(L-Lactic Acid) Nanotopography Edge Density and Proximity,” Int. J. Nanomedicine, 2(2), pp. 201–211. [PubMed]
Weston, M. W., Laborde, D. V., and Yoganathan, A. P., 1999, “Estimation of the Shear Stress on the Surface of an Aortic Valve Leaflet,” Ann. Biomed. Eng., 27, pp. 572–579. [CrossRef] [PubMed]
Sato, T., Yamaguchi, H., Shinmura, T., and Okazaki, T., 2006, “Study of Surface Finishing Process Using Magneto-Rheological Fluid (MRF)—2nd Report: Effects of the Finishing Behavior of MRF-Based Slurry on Finishing Characteristics,” J. Jpn. Soc. Precis. Eng., 72(11), pp. 1402–1406 (in Japanese).
Yamaguchi, H., Shinmura, T., and Kobayashi, A., 2001, “Development of an Internal Magnetic Abrasive Finishing Process for Nonferromagnetic Complex Shaped Tubes,” JSME Int. J., Ser. C, 44(1), pp. 275–281. [CrossRef]
Kidane, A. G., Burriesci, G., Cornejo, P., Dooley, A., Sarkar, S., Bonhoeffer, P., Edirisinghe, M., and Seifalian, A. M., 2009, “Current Developments and Future Prospects for Heart Valve Replacement Therapy,” J. Biomed. Mater. Res., Part B: Appl. Biomater., 88B(1), pp. 290–303. [CrossRef]
Daniels, A. U., 2012, “Silicone Breast Implant Materials,” Swiss Med. Wkly, 142, w13614. [PubMed]
Roe, B. B., Kelly, P. B., Myers, J. L., and Moore, D. W., 1966, “Tricuspid Leaflet Aortic Valve Prosthesis,” Circulation, 33, pp. 124–127. [CrossRef] [PubMed]
Kannan, R. Y., Salacinski, H. J., Ghanavi, J., Narula, A., Odlyha, M., Peirovi, H., Butler, P. E., and Seifalian, A. M., 2007, “Silsesquioxane Nanocomposites as Tissue Implants,” Plast. Reconstr. Surg., 119(6), pp. 1653–1662. [CrossRef] [PubMed]
Ghanbari, H., Kidane, A. G., Burriesci, G., Ramesh, B., Darbyshire, A., and Seifalian, A. M., 2010, “The Anti-Calcification Potential of a Silsesquioxane Nanocomposite Polymer Under In Vitro Conditions: Potential Material for Synthetic Leaflet Heart Valve,” Acta Biomater., 6, pp. 4249–4260. [CrossRef] [PubMed]
Rahmani, B., Tzamtzis, S., Ghanbari, H., Burriesci, G., and Seifalian, A. M., “Manufacturing and Hydrodynamic Assessment of a Novel Aortic Valve Made of a New Nanocomposite Polymer,” J. Biomech., 45(7), pp. 1205–1211. [CrossRef] [PubMed]
Goodman, S. L., 1999, “Sheep, Pig, and Human Platelet—Material Interactions With Model Cardiovascular Biomaterials,” J. Biomed. Mater. Res., 45, pp. 240–250. [CrossRef] [PubMed]
Chung, S., Im, Y., Kim, H., Jeong, H., and Dornfeld, D. A., 2003, “Evaluation of Micro-Replication Technology Using Silicone Rubber Molds and Its Applications,” Int. J. Mach. Tools Manuf., 43(13), pp. 1337–1345. [CrossRef]
Yamaguchi, H., and Hanada, K., 2008, “Development of Spherical Magnetic Abrasive Made by Plasma Spray,” ASME J. Manuf. Sci. Eng., 130, p. 031107. [CrossRef]

Figures

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Fig. 1

Photographs of porcine pulmonary valve and polymeric valve

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Fig. 2

Average thickness of silicone leaflets with mold revolution

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Fig. 3

Processing principle and finishing machine

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Fig. 4

Mold surface roughness profiles

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Fig. 5

Leaflet roughness Sa with mold roughness Ra

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Fig. 6

Three-dimensional surface shapes of mold 4a and leaflet 4a measured by optical profiler

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Fig. 7

Three-dimensional surface shapes of leaflet 6 cured in air (0.42 μm Sz, 0.07 μm Sa)

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Fig. 8

Blood cell adhesion test schematic and photograph of experimental setup

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Fig. 9

Microscopic images (170 μm × 220 μm) of blood cell adhesion on leaflet 2 (with grid)

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Fig. 10

Average number of platelets adhered to silicone leaflets

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Fig. 11

Relationship between average numbers of platelets and red blood cells adhered to leaflet and leaflet roughness

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Fig. 12

Relationship between average numbers of platelets and red blood cells adhered to leaflet and mold roughness

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