Special Section Technical Briefs

Visualizing Vascular Structure in the Adult Newt (Notophthalmus viridescens) Heart1

[+] Author and Article Information
Bhairab N. Singh, Cyprian Weaver, Naoko Koyano-Nakagawa, Daniel J. Garry

Lillehei Heart Institute,
University of Minnesota,
Minneapolis, MN 55455

Accepted and presented at The Design of Medical Devices Conference (DMD2015), April 13-16, 2015, Minneapolis, MN, USA.

Manuscript received March 3, 2015; final manuscript received March 16, 2015; published online April 24, 2015. Editor: Arthur Erdman.

J. Med. Devices 9(2), 020917 (Jun 01, 2015) (2 pages) Paper No: MED-15-1052; doi: 10.1115/1.4030125 History: Received March 03, 2015; Revised March 16, 2015; Online April 24, 2015

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Grahic Jump Location
Fig. 1

Absence of coronary vessels in the newt heart. (a) Frontal whole-mount view of newt heart. (b) H&E staining showing AT, highly trabeculated ventricle (V), and BA. (c) Silver nitrate staining in the newt tail showing deposition of silver in the endothelial lining of the blood vessels. (d)–(f) Silver nitrate staining in the newt heart revealed absence vessel-like structures.

Grahic Jump Location
Fig. 2

Electron microscopic analysis of the newt heart. (a) Low power SEM image, showing the ventral view of newt heart. (b) and (c) High power SEM images of the ventricular area revealing tightly connected outer cell layer and tiny projections. (d) and (e) Sagittal section of the newt heart revealing inner structures. Note the distinct outer epicardial layer and highly trabeculated myocardial layer forming spongy-network. Nucleated red blood cells can also be seen as disklike structures.




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