The purpose of this study was to evaluate the suitability of a novel radio-frequency identification (RFID)-based tracking system for intraoperative magnetic resonance imaging (MRI). A RFID tracking system was modified to fulfill MRI-compatibility and tested according to ASTM and NEMA. The influence of the RFID tracking system on MRI was analyzed in a phantom study using a HASTE and TrueFISP sequence. The RFID antenna was gradually moved closer to the isocenter of the MR scanner from 90 to 210 cm to investigate the influence of the distance. Furthermore the RF was gradually changed between 865-869 MHz for a distance of 90 cm, 150 cm and 210 cm to the isocenter of the magnet to investigate the influence of the frequency. The specific spatial resolution was measured with and without a permanent line of sight (LOS). After the modification of the reader no significant change of the SNR could be observed with increasing distance of the RFID tracking system to the isocenter of the MR scanner. Also different radio frequencies of the RFID tracking system did not influence the SNR of the MR images significantly. The specific spatial resolution deviated on average by 8.97 ± 7.33 mm with LOS and 11.23 ± 12.03 mm without LOS from the reference system. The RFID tracking system had no relevant influence on the MR image quality. RFID tracking solved the LOS problem. However the spatial accuracy of the RFID tracking system has to be improved for medical usage.