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Technical Brief

Effect of the Bowl Structure in an Automated Cell-Isolation Device on Stromal Vascular Fraction's Isolation Yield

[+] Author and Article Information
Hyung Min Hahn

Department of Plastic and Reconstructive Surgery,
Ajou University Hospital,
164 World cup-ro,
Yeongtong-gu 16499, Suwon, South Korea
e-mail: newcetizen@naver.com

Kwang Sik Jeong

Department of Plastic and Reconstructive Surgery,
Ajou University Hospital,
164 World cup-ro,
Yeongtong-gu 16499, Suwon, South Korea
e-mail: jks8486@aumc.ac.kr

Bo Young Yoo

Medical and Scientific Affairs Team,
Research Center, CGBIO Co., Ltd,
12, Bongeunsa-ro 114-gil,
Gangnam-gu 06170, Seoul, South Korea
e-mail: by8296@cgbio.co.kr

Jong Ha Park

Research Center, CGBIO Co., Ltd,
Seongnam-si 462-120, Gyeonggi-do, South Korea
e-mail: jpark@cgbio.co.kr

Hyun Joo Jung

Department of Pediatrics and Adolescent Medicine,
Ajou University Hospital,
164 World cup-ro,
Yeongtong-gu 16499, Suwon, South Korea
e-mail: free1109@ajou.ac.kr

Il Jae Lee

Department of Plastic and Reconstructive Surgery,
Ajou University School of Medicine,
164 World cup-ro,
Yeongtong-gu 16499, Suwon, South Korea
e-mail: i00325@live.co.kr

1Corresponding author.

Manuscript received January 31, 2018; final manuscript received August 7, 2018; published online September 21, 2018. Assoc. Editor: Yaling Liu.

J. Med. Devices 12(4), 044501 (Sep 21, 2018) (5 pages) Paper No: MED-18-1023; doi: 10.1115/1.4041191 History: Received January 31, 2018; Revised August 07, 2018

The enzymatic digestion of lipoaspirate is used to isolate the heterogeneous stromal vascular fraction (SVF) that contains the adipose-derived stromal cells (ASCs). Several automated SVF isolation systems are used to operate standard technical procedures and avoid human errors. However, the yield of isolated cells and the residual collagenase activities of the SVF samples obtained from automated systems are not satisfactory compared to those from manual isolation methods. In this study, we evaluated the efficiency and the reliability of a new automated SVF isolation system in which the bowl was designed in the shape of a radial protrusion at each angle (a top-type bowl). The viability and yield of cells and the residual collagenase activities of SVFs obtained in a top-type bowl were compared with the SVFs obtained in a conventional bowl. We achieved a significantly higher yield of cells and decreased residual collagenase activity in the SVFs obtained from a top-type bowl (18.0 × 105 cells/mL of fat) compared to a conventional bowl (2.3 × 105 cells/mL). There was no significant difference in the cell viability between the two groups. These results suggest that the automated SVF isolation system with an improved bowl structure will potentially yield higher numbers of nucleated cells and decreased residual collagenase activity compared to conventional automated systems in cell-based clinical trials.

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Figures

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Fig. 1

A cannula for tumescent infiltration (upper) and power-assisted liposuction handpiece (lower), which provides power to reciprocate a cannula back and forth during procedure

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Fig. 2

Automated SVF-isolation device (cellunit) used in this study (a) and the procedure of SVF isolation which the cellunit device simulated (b)

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Fig. 3

The disposable kit of a cellunit device containing a bowl and their connections

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Fig. 4

Variations of centrifugal efficacy by bowl structures, conventional type bowl (a) and top-type bowl (b). The top-type bowl is structured with a slope to collect cells from every part of the cylinder by centrifugal forces during rotation.

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Fig. 5

The average numbers of SVF cells isolated from the conventional bowl and top-type bowl were 2.3 × 105 cells/mL and 18.0 × 105 cells/mL of lipoaspirate, respectively (a). There was a significant difference between two groups (P < 0.001). Cell viabilities isolated from the conventional bowl and top-type bowl were 84.1% and 80.6%, respectively (b). There was no significant difference between two groups (P = 0.375).

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Fig. 6

Residual collagenase activities (a) and ratios (b) of final SVF supernatants, which were determined with an Enzchek assay kit. There were significant differences of residual collagenase activities and ratios between two different groups (P < 0.001).

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